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KMID : 1140120110160040358
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Cancer Prevention Research
2011 Volume.16 No. 4 p.358 ~ p.370
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Induction of Apoptosis by Bojungikgigilkyung-tang in Human Lung Cancer A549 Cells
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Bae Jong-Hoon
Shin Dong-Yeok
Park Cheol
Choi Yung-Hyun
Park Dong-Il
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Abstract
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Bojungikkigamikilkyung-tang (BJIKTP) is a traditional oriental herbal formulation consisting of crude ingredients extracted from nine herbs. This prescription has been widely used for patients suffering from a lung disease in Oriental medicine. In the present study, it was examined the biochemical mechanisms of apoptosis induced by BJIKTP in human non-small-cell lung cancer (NSCLC) cells. It was found that exposure to BJIKTP was found to result in a concentration-dependent growth inhibition by induction of apoptosis, as evidenced by chromatin chondensation, DNA fragmentation and the accumulation of sub-G1 phase, in NSCLC A549 cells, but not in NCI-H460 cells. Apoptosis induction of A549 cells by BJIKTP was connected with a modulation of Bcl-2 and inhibitors of apoptosis (IAP) family members, and up-regulation of death-receptor related members. BJIKTP treatment also induced proteolytic activation of caspase-3, -8 and -9, and degradation of caspase-3 substrate proteins including poly (ADP-ribose) polymerase and phospholipase C γ-1. In addition, BJIKTP-induced apoptosis is associated with the reduction of mitochondrial membrane potential (MMP) and cleavage of Bid proteins. Taken together, the results indicate that BJIKTP may be a potential chemotherapeutic agent for use in the control of human lung cancer and that further studies are needed for the identification of the active compounds.
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KEYWORD
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BJIKTP, A549, Apoptosis, Caspase, Bid
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